quantikine human il 6sr immunoassay kit  (R&D Systems)

Journal: PLoS ONE

Article Title: Human Adipocytes Stimulate Invasion of Breast Cancer MCF-7 Cells by Secreting IGFBP-2

doi: 10.1371/journal.pone.0119348

Figure Lengend Snippet: 3A. MCF-7 cells were digested to put into transwells after incubation with IGFBP-2 (680 pg/ml), PDGF-BB (210 pg/ml), IL-6sR (110 pg/ml) and TNF-a (529 pg/ml) separately for 24 hours. IGFBP-2 enhanced the invasion ability of breast cancer cells most prominently at 680 pg/ml. 3B. We added IGFBP-2 to normal DMEM at 680 pg/ml and called it IGFBP-2-CM. CAA-CM and IGFBP-2-CM induced higher MMP-2 expression in MCF-7 cells than did C-CM. IGFBP-2 was not elevated in MCF-7 cells treated with CAA-CM at the mRNA or protein level. 3C. Medium obtained from the co-culture with IGFBP-2 knock-out adipocytes did not induce the invasion of the cancer cells, whereas the medium obtained from the co-culture of IGFBP-2 knock-out breast cancer cells were able to induce invasion (*: P<0.05, **: P<0.01, ***: P<0.001).

Article Snippet: Recombinant human IGFBP-2, PDGF-BB, IL-6sR and TNF-a were purchased from Peprotech and dissolved in auxiliary solvent.

Techniques: Incubation, Expressing, Co-Culture Assay, Knock-Out

Journal: PLoS ONE

Article Title: Human Adipocytes Stimulate Invasion of Breast Cancer MCF-7 Cells by Secreting IGFBP-2

doi: 10.1371/journal.pone.0119348

Figure Lengend Snippet: Primers used for Q-PCR amplification of human mature adipocytes.

Article Snippet: Recombinant human IGFBP-2, PDGF-BB, IL-6sR and TNF-a were purchased from Peprotech and dissolved in auxiliary solvent.

Techniques: Amplification